Are you sometimes unable to reproduce results that relied on antibodies?

Are you sometimes unable to reproduce results that relied on antibodies?

Are you sometimes unable to reproduce results that relied on antibodies?

Have you been struggling with research involving antibodies? In a recent article in Nature,Monya Baker highlights the current trend of failure to reproduce research involving antibodies. Baker alertsto some of the most common pitfalls that researchers should be aware of, and provides hints how this could be avoided. These include the necessity to validate antibodies purchased from reagent companies, the potentially devastating effects of untrustworthy antibodies on research findings, and the incorrect application of antibodies. The article stresses the importance of purchasing antibodies from reputable companies, not those able to provide the cheapest product or with fastest delivery time. It also provides information about available resources to ensure that antibodies to be employed comply with what actually required.

Extract of article completed by Dr. Marlise van Staden (University of Limpopo):

Baker, M. 2015. Reproducibility crisis: Blame it on the antibodies. Nature521, 274 – 276 (21 May 2015).

Download full article here:
Blame it on the antibodies Nature May 2015